薏苡愈伤组织的诱导与分化

Calli Induction and Differentiation of Coix lachryma-jobi

为薏苡优良品种的脱毒快繁和基地化脱毒种苗的生产提供科学依据,以薏苡气生根、种子胚、嫩茎为材料,研究了2,4-D、KT、NAA不同浓度配比对愈伤组织的诱导、分化和成苗的影响。结果表明,KT是薏苡芽分化及脱毒种苗生成的主要影响因素,气生根是诱导胚性愈伤组织及胚状体最合适的外植体,其最佳分化培养条件为MS+2,4-D 0.4mg/L+KT 1.5mg/L+NAA 0.8mg/L,最佳成苗培养基为MS+2,4-D 0.4mg/L+KT 1.5mg/L+NAA 1.0mg/L。

To provide scientific basis for the detoxication and rapid propagation of the fine variety of C.lachrymal-jobi and the production of virue-free plantlets in plant area,the effects of different concentrations of mixed 2,4-D,KT,NAA on callus induction,differentiation and seedling was researched.The materials of this research were aerial root of C.lacryma-jobi,seed embryo and spears.The results showed that KT was the main factor affecting the differentiation of C.lacryma-jobi spears and the generating of the virus-free plantlets.Aerial root was the best explants for inducing embryonic callus and embryoid and its optimal differentiation culture condition was MS ＋2,4-D 0.4 mg/L＋ KT 1.5 mg/L＋NAA 0.8 mg/L,its seedling culture medium was MS ＋2,4-D 0.4 mg/L＋ KT 1.5 mg/L＋NAA 1.0 mg/L.