摘要
为探索纤维素酶和木聚糖酶降解艾蒿纤维素的最佳条件,以利于研究后续的乙醇发酵生产工艺,研究了不同因素对纤维素酶和木聚糖酶降解艾蒿粉的影响。结果表明:双酶降解艾蒿纤维素的最佳工艺条件为温度40℃、时间40h、pH 4.60、磷酸盐缓冲液4mL和双酶配比1∶1。
The influences of pH,time,temperature,phosphate buffer dosage,metal ion concentration and the ratio of cellulose to xylanase on degradation of cellulose from A.argyi were studied to explore the optimum degradation conditions and to be beneficial to further study of productive technology of alcoholic fermentation.The results showed that the best conditions were as follows: temperature 40℃,time 40 h,pH 4.60,PBS dosage 4 mL,cellulose-xylanase ratio 6∶6(namely 1∶1).
出处
《贵州农业科学》
CAS
北大核心
2012年第5期169-172,共4页
Guizhou Agricultural Sciences
基金
河南省科技厅基础与前沿项目"生物乙醇原料的开发与利用"(092300410083)
关键词
纤维素酶
木聚糖酶
艾蒿
纤维素
工艺优化
cellulose
xylanase
Artemisia argyi
cellulose
process optimization