纤维素酶和木聚糖酶降解艾蒿纤维素工艺的优化

Optimization of Degradation of Cellulose from Artemisia argyi by Cellulose and Xylanase

为探索纤维素酶和木聚糖酶降解艾蒿纤维素的最佳条件,以利于研究后续的乙醇发酵生产工艺,研究了不同因素对纤维素酶和木聚糖酶降解艾蒿粉的影响。结果表明:双酶降解艾蒿纤维素的最佳工艺条件为温度40℃、时间40h、pH 4.60、磷酸盐缓冲液4mL和双酶配比1∶1。

The influences of pH,time,temperature,phosphate buffer dosage,metal ion concentration and the ratio of cellulose to xylanase on degradation of cellulose from A.argyi were studied to explore the optimum degradation conditions and to be beneficial to further study of productive technology of alcoholic fermentation.The results showed that the best conditions were as follows： temperature 40℃,time 40 h,pH 4.60,PBS dosage 4 mL,cellulose-xylanase ratio 6∶6（namely 1∶1）.