基于SPR技术研究壳聚糖对Pb^(2+)的吸附动力学

Study on Adsorption Kinetics of Chitosan for Lead Based on SPR

采用自主构建的表面等离子共振(SPR)生物传感系统研究了壳聚糖对Pb2+的吸附动力学。通过双官能团偶联剂将壳聚糖固定在传感器芯片表面,利用壳聚糖分子中含有的大量活性基团与Pb2+配位形成金属螯合物,研究了壳聚糖对不同浓度Pb2+的吸附过程,并进行动力学分析。结果表明,壳聚糖对铅离子的吸附符合Langmuir单分子吸附理论所提出的动力学方程,为壳聚糖在处理含铅环境污染物方面提供了一种理论依据。

Adsorption kinetics of chitosan for Pb^2 ＋ was studied based on surface plasmon resonance （SPR） biosensor system, which was built by our group. Chitosan was first immobilized on the surface of sensor chip by a bifunctional cross-linker. The metal chelates can be formed between Pb^2＋. and chitosan molecules with a large number of active groups. The adsorption process and kinetics of chitosan for binding the lead at different concentrations were investigated. The results showed that the adsorption kinetics of chitosan for Pb^2＋ was corresponded to the adsorption theory of Langmuir monolayer, which provided a theoretical basis in dealing with lead in environment pollutants.