摘要
目的:建立大血藤药材中绿原酸的含量测定方法。方法:采用高效液相色谱法,Diamonsil ODS-C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-4%磷酸水(11∶89),流速1.0 mL.min-1,检测波长327 nm,柱温25℃。结果:绿原酸在0.000 102～0.010 2 mg线性关系良好。加样回收率为102.4%,RSD 1.7%(n=6)。结论:采用HPLC测定大血藤药材中绿原酸的含量,样品处理方法便捷,测定方法简单,结果准确可靠,可用于大血藤饮片、大血藤制剂的质量控制。
Objective： To establish the determination method of chlorogenic acid in Sargentodoxa cuneata. Method： HPLC was used with Diamonsil ODS-Cls column （4.6 mm 250 mm, 5 μm） as chromatographic column and acetonitrile-4% phosphoric acid solution （11：89） as mobile phase. The flow rate was 1.0 mL min-l; the detection wavelength was set at 327 nm and the column temperature was kept at 25 ℃ Result： The linear range of chlorogenic acid was 0. 000 102-0. 010 2 mg. The average recovery was 102.57% , RSD 0. 83% （n = 6）. Conclusion： The method of sample processing was simple and the HPLC method was accurate and reliable. It can be used for the quality control of S. cuneata and its preparation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第4期99-102,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市科委资助项目(09dZ1975500)
上海市人才发展基金项目(20111015)
上海市教委基金项目(10SG40
J50302)
关键词
绿原酸
高效液相色谱法
含量测定
大血藤
chlorogenic acid
HPLC
content determination
Sargentodoxa cuneata
