摘要
目的:应用高效液相色谱法测定长春西汀注射液的有关物质。方法:采用Hypersil ODS C18色谱柱(4.6 mm×150 mm,5μm),甲醇-1.75 g.L-1碳酸铵溶液-乙酸(80∶25∶3)为流动相,流速1.0 mL.min-1,检测波长273 nm。结果:在选定的色谱条件下,长春西汀与有关物质分离完全,最低检测限为20 ng,精密度良好(RSD 0.52%)。结论:方法简便、准确、专属性好、灵敏度高,可用于长春西汀注射液的有关物质测定。
Objective： To determine the related substances of Vinpocetine Injection by HPLC. Method： The separation was performed on Hypersil ODS Cls column （4.6 mm x 150 mm, 5 Ixm）. The mobile phase consisted of methanol-1.75 g .L-1 ammonium carbonate solution-acetic acid （80：25：3 ）, the flow rate was set at 1.0 mL-min-lwith detection at 273 rim. Result： Under the established chromatographic conditions, the related substances and vinpocetine were separated completely. The detective limit was 20 ng. The precision in a day was good （RSD 0.52% ）. Conclusion： The method is simple, accurate, specific and sensitive for the determination Vinpocetine Injection related substances.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第4期137-139,共3页
Chinese Journal of Experimental Traditional Medical Formulae
