7种中草药提取物抗过敏功效及刺激性研究

Experimental Study on Anti-allergic Effect of Extracts from Seven Kinds of Chinese Herbal Medicine

目的:考查地肤子皂苷、防风多糖、桃仁和金银花水提物、茶多酚、洋甘菊和仙人掌提取物的抗皮肤过敏作用,探究其刺激性大小,为作为具有抗过敏功效化妆品添加剂提供依据。方法:采用透明质酸酶体外抑制试验和红细胞溶血试验,对7种中草药提取物抗过敏特性及其刺激性进行探究。结果:7种中草药有效部位或成分的抗过敏抑制率分别为洋甘菊提取物(77.04%)>金银花水提物(69.54%)>茶多酚(68.01%)>仙人掌提取物(62.67%)>防风多糖(55.51%)>地肤子皂苷(43.09%)>桃仁水提物(32.74%);其中只有茶多酚添加浓度超过6 g.L-1时显示出一定的刺激性,其他有效成分的溶血率均低于20%,刺激性很小。结论:7种中草药有效部位或成分均表现一定的抗过敏特性,且在规定添加量范围内刺激性均很小,适合作为抗过敏化妆品添加剂使用。

Objective:To investigate the anti-allergic effect of extracts from Fructus Kochia Scoparia,Saposhnikovia divaricata,Peach seed,Lonicera,tea polyphenols,Chamomile Extractand and Opuntia,to evaluate their irritating effect,and to provide data for the herbal medicine as potential additives in cosmetic.Method:The method of hyaluronidase inhibition in vitro was used to explore the anti-allergic properties of the extracts of the seven Chinese herbal medicine.And hemolysis of red blood cell was used to evaluate their irritating effects.Result:The anti-allergy inhibition rates of the extracts were as following:Chamomile Extract(77.04%)>Lonicera water extract(69.54%)>tea polyphenols(68.01%)>Opuntia extract(62.67%)>S.divaricata(55.51%)>Fructus Kochia Scoparia(43.09%)>Peach seed water extract(32.74%).In the seven extracts,only tea polyphenols(concentration over 6 g · L-1) showed certain irritation,hemolysis rates of other extracts were lower than 20%,so irritation was very light.Conclusion:Extracts from seven kinds of Chinese herbal medicine show certain anti-allergic properties.And within a predetermined addition amount,their irritating can be very light.So they might be used as allergy cosmetic additives.