滋阴清热方对金黄地鼠皮脂腺增生的影响

Effect of Ziying Qingre Prescription on Proliferation of Sebaceous Gland in Golden Hamster

目的:探讨滋阴清热方治疗寻常痤疮的机制。方法:选用成年雄性金黄地鼠侧腹部皮脂腺斑作为动物模型,随机分为4组,分别予生理盐水、安体舒通(10 mg.kg-1)、滋阴清热方高剂量(18 g.kg-1)、滋阴清热方低剂量(9 g.kg-1)灌胃给药,每天1次,连续4周。实验前电动剃须刀剃尽双侧腹部毛,充分暴露皮脂腺斑。实验前后强光下,用游标卡尺测量双侧皮脂腺斑的最大横径和最大纵径。观察用药前后皮脂腺斑大小(最大横径×最大纵径)变化及行HE染色对皮脂腺斑组织形态学进行观察。结果:清热滋阴方高剂量组双侧皮脂腺斑大小与空白组比较有显著性差异(P<0.05),与阳性药组比较无统计学差异。组织学上变化明显,滋阴清热方能使皮脂腺腺体层数减少,腺体萎缩,排列松散。结论:滋阴清热方具有抑制皮脂腺增生的作用,可能是其治疗寻常性痤疮的机制之一。

Objective： In this study, we aim to investigate mechanism of Ziying Qingre prescription on acne. Method： The animals were randomly divided into 4 groups, namely blank control group, positive control group, high and low dosage of Ziying Qingre prescription. They were respectively fed with saline solution, antisterone （10 mg kg-1）, Ziying Qingre prescription （18, 9 g .kg-1） for 4 weeks. We measured the greatest transverse and greatest longitudinal diameter of bilateral sebaceous gland plaques with vernier caliper under strong light. The size change （greatest transverse diameter multiply greatest longitudinal diameter） and histopathology of sebaceous gland plaques were observed. Result： The size of sebaceous gland plaques of high dosage group were decreased （P 〈 O. 05） compared to the blank control groupHistopathology showed Ziying Qingre prescription made the numbers of sebaceous gland layers decreased, gland atrophy, arrangmentloose. Conclusion： Ziying Qingre prescription can suppress the proliferation of sebaceous glandwhich may be one of the mechanisms in treatment of acne.