光叶娃儿藤解热抗炎作用实验研究

Studies on Antipyretic and Anti-inflammatory Effects of Tylophora ovata

目的:观察光叶娃儿藤的解热和抗炎作用。方法:SD大鼠随机分为生理盐水对照组,阿司匹林组(0.1 g.kg-1),光叶娃儿藤低、中、高剂量组(6,12,24 g.kg-1)。分别采用酵母混悬液复制大鼠发热模型和角叉菜胶致大鼠足趾肿胀法,观察光叶娃儿藤的解热和抗炎作用,同时紫外分光光度法测定大鼠角叉菜胶致炎足渗出物中的前列腺素E2(PGE2)的含量。结果:与生理盐水组比较,光叶娃儿藤中、高剂量组(12,24 g.kg-1)能显著降低酵母菌致大鼠发热模型的体温(P<0.05),并能显著抑制角叉菜胶致大鼠的足肿胀度(P<0.05)和足组织中的前列腺素E2(PGE2)的合成(P<0.05)。结论:光叶娃儿藤具有明显的解热和抗炎作用,其机制可能与抑制PGE2等炎症介质合成有关。

Objective： To explore the antipyretic and anti-inflammatory effects of Tylophora ovata. Method： SD rats were divided into 5 groups randomly： physiological saline group, positive control group, the water extract of T. ovata low-dose group （6 g kg-1） , middle-dose group （12 g kg-1） and high-dose group （24 g kg-L）. The fever model with high temperature was established induced by yeast in rats, and the inflammatory model with foot edema was established by carrageenan in rats. The antipyretic and the anti-inflammatory effects of T. ovata were observed, the content of prostaglandin E2 （PGE2 ） in paw tissue were tested by ultraviolet spectrophotometry. Result： Compared with the saline group, the water extract of T. ovata middle-dose group and high-dose group showed significant antipyretic activity in rats with high temperature induced by yeast （P 〈 0. 05） , and appeared significant anti-inflammatory activity in rats with foot edema induced by carrageenin （P 〈 0.05 ）. Conclusion： T. ovata had notable effects of antipyretie and anti-inflammatory, the anti-inflammatory mechanism might be related to inhibition of PGE2 biosynthesis.