九里香指纹图谱与其抗炎活性的灰关联度分析

Gray Relational Analysis on Fingerprint of Murraya exotica and Anti-Inflammation Effects

目的:建立中药九里香HPLC指纹图谱,确定九里香不同萃取部位的指纹图谱特征峰与其抗炎活性作用贡献大小。方法:利用HPLC研究中药九里香指纹图谱及不同萃取部位的指纹图谱;利用角叉菜胶诱导小鼠足肿胀试验研究其抗炎作用;利用灰关联度分析方法研究其谱效关系。结果:依照10批次九里香样品的色谱图构建九里香药材的HPLC指纹图谱,确定了17个共有色谱峰,相似度均>0.90。九里香不同萃取部位的抗炎作用是多种化学成分共同作用的结果,各特征峰对其抑制足肿胀作用贡献大小顺序为9>7>2>1>10>5;对下调血清中前列腺素(PGE2)活性的贡献大小顺序为5>10>7>9>4。结论:所构建的九里香HPLC指纹图谱方法简单,重复性好,可为九里香的鉴别和质量评价提供参考。九里香不同萃取部位指纹图谱与其抗炎作用之间有一定的对应关系。

Objective ： To establish of the fingerprint peaks from different solvents the PLC extracts fingerprint of Murraya exotica, to anti-inflammation. Method ： to determine the contribution The HPLC fingerprints of M exotica and different solvents extracts were established; mice paw edema model induced by carrageenan was used to study the anti-inflammation activity, and gray relational analysis was used to study the fingerprint-effect relationship. Result： The HPLC fingerprint of M. exotica was established from 10 batches of M. exotica samples, which had identified 17 common peaks, with similarity of more than 0.90. The anti-inflammative activity of different solvents extracts was obtained from many constituents collaboratively. The contribution of different peaks to inhibit the mice paw edema was ranked with 9 〉 7 〉 2 〉 1 〉 10 〉 5, and 5 〉 10 〉 7 〉 9 〉4 for down-regulation of the serum PGE2 content. Conclusion ： The method for establishing HPLC fingerprint of M. exotica is simple, repeatable, which can provide the information for identification and quality evaluation of M. exotica. The relationship between the fingerprint of different solvents extracts from M. exotica and anti-inflammation suggests to be paralleled to some extent.