拟南芥尾翼茎突变体tfos基因的分子标记定位

Chromosomal Location of Tail-Fins-on-Stem Gene in Arabidopsis thaliana

在短日照生长条件下,拟南芥维管发育有一定量的次生生长,可模拟林木木材的形成过程。前期研究中,筛选到 1 个突变体,在短日照生长条件下,相对于野生型,该突变体植株矮化且茎中下部有脊状结构附着,并伴随有发育迟缓、营养生长时期延长和莲座叶叶片边缘呈锯齿状等性状,将其命名为尾翼茎突变体( tfos) 。切片显微观察表明,尾翼组织具有明显维管结构,推测为茎内部细胞不正常分化导致该表型; 遗传分析显示,突变性状受隐性单基因控制。进一步利用分子标记技术对该基因进行定位分析,结果将其定位到 1 号染色体上,与 SSLP 标记F11A17-48074 紧密连锁。

Arabidopsis thaliana undergoes to a certain degree secondary growth under short day conditions,thus it can be used to study wood formation.In our previous work,we screened a mutant(tail-fins-on-stem,tfos) that displayed unique characteristics under short day conditions,such as shorter stem with a few ridge-like structures on the middle and the basal stem,slower growth and twisted rosette leaves with serrated margin,in comparison with the wild type.Microscopic observation showed that there was vascular structure in the middle of the ridge,likely due to abnormal differentiation of the cells in the stem.Genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene.Further analysis,with molecular markers,showed that the mutant gene was mapped on the Chromosome I and displayed co-separation with the SSLP marker F11A17-48074.This study paved a way for cloning the gene controlling the phenotypes.