转Bt基因南林895杨对杨扇舟蛾体内酶的影响

Effects of Bt Nanlin 895 Poplar on the Metabolic Enzymes of Scarce Chocolate Tip,Clostera anachoreta

以转Bt基因南林895杨树叶片饲喂杨扇舟蛾,研究 Bt 毒蛋白对害虫体内代谢酶的影响。结果表明: 酯酶和羧酸酯酶明显受到抑制。中肠酯酶活性在取食 4 ～ 48 h 内持续下降,羧酸酯酶活性在前期 4 h 有所上升但随后持续下降。多功能氧化酶活性从 24 h 起,受抑而下降,但不显著。谷胱甘肽 S - 转移酶活性在前 36 h 内波动不大,48 h 后显著受抑。乙酰胆碱酶的活性无明显受抑。超氧化物歧化酶活性 12 h 后略升高,然而在取食 12 ～ 48 h活性持续下降,与之相反,过氧化物酶活性在 4 ～ 48 h呈上升状态。过氧化氢酶活性在 4 ～ 12 h 呈下降状态,24 ～48 h呈上升状态。转 Bt 基因杨树主要通过抑制酯酶和羧酸酯酶并扰乱超氧化物歧化酶、过氧化氢酶、过氧化物酶的协调作用干扰害虫的正常生理代谢,起到杀虫作用。

The larvae of scarce chocolate tip,Clostera anachoreta,were fed with leaves of Bt Nanlin 895 poplar to study effects of Bt protein on the metabolic enzymes of the pest.The results showed that the activities of esterase and carboxylesterase were inhibited by Bt protein.The esterase activity of the larvae reduced after being fed with the transgenic poplar leaves in 4-48 h and the activity of carboxylesterase rose during the initial 4 h but declined after then.The activity of multi-function oxidase in the larvae slightly declined after being fed with the transgenic leaves in 24 h,but the difference was not significant from CK.The activity of glutathione-S-transferase did not significantly change during the initial 36 h,but was inhibited by the Bt protein in 48 h.The activity of acetylcholinesterase was not affected by the feeding.The activity of superoxide dismutase rose in 12 and then declined during 12-48 h.The activity of peroxidase rose during 4-48 h while the activity of catalase declined during 4-12 h and rose from 24-48 h.Therefore,the transgenic poplar inhibited the activities of esterase and carboxylesterase,disturbed the protect enzyme system and had an insecticidal effect of the larvae.