桉树内生菌对尾巨桉幼苗抗寒生理指标的影响

Cold Resistance Nutrient Characters with Ten Kinds of Fungus in Eucalyptus urophylla × E.grandis

20世纪50年代我国开始大规模种植桉树（Eucalyptus spp.）,现在栽植已经遍布我国17个省、自治区的600多个县,栽植面积达到150多万hm^2（祁述雄,2002）。然而,随着桉树大面积的种植,影响其种植的主要限制因子低温伤害也逐渐呈现。目前对桉树抗寒性的研究主要有抗寒锻炼、良种选育与引种、抗寒分子水平的研究等方面（庄国庆等,2010;赵娟娟,2006;郭祥泉,2003;黄德先等,

E.urophylla × E.grandis seedlings were inoculated with 10 strains of eucalyptus endophytic fungi by irrigating the fungus suspension,and then subjected to low temperature stress 30 days later.The results showed that compared with the control the soluble sugar(SS) content increased by 41.11%,11.11%,32.78%,38.89% and 6.67% in treatment 1,8,5,7,10,respectively.The soluble protein(SP) content was 14.90%,0.72%,10.94%,3.37%,24.40% and 0.36% higher in treatment 1,2,5,6,7,10 than that in the control.The proline content was 28.77%,22.11%,20.16%,11.25%,5.48% and 1.57% higher in treatment 5,7,1,2,4,9 than that in the control.The chlorophyll content was 9.52%,7.48%,7.48%,6.80%,5.44%,3.40%,3.40%,2.72%,2.04% and 2.04% higher in treatment 6,8,10,1,9,5,7,4,2,3 than that in the control.After being inoculated with strain 1 of the fungus,the cold-resistant capability of eucalyptus was much improved in terms of nutrition characteristics.However,the more physiological characteristics may be needed to determine whether the fungus can be applied in production.