乳化异氟醚后处理对成年大鼠缺氧/复氧心肌细胞超微结构的影响

The effect of emulsified isoflurane postconditioning on myocardial ultrastructures of adult rats with hypoxia/reoxygenation injury

目的观察乳化异氟醚(emulsified isofurane,EI)对原代培养成年大鼠心肌细胞缺氧/复氧损伤后超微结构的影响。方法体外培养成年大鼠心肌细胞,建立心肌细胞缺氧/复氧损伤模型,然后将心肌细胞随机分为7组,正常组(N组)、缺氧/复氧组(H/R组)、缺氧后处理组(HPO组)和不同浓度EI后处理组(0.84mmol/L、1.68mmol/L和2.52mmol/L,EI1～EI3组)及脂肪乳组(F组),倒置显微镜观察心肌细胞生长的特性及形态的变化;采用透射电子显微镜观察缺氧/复氧损伤后心肌细胞超微结构的变化。结果心肌细胞电镜显示正常组心肌细胞超微结构正常,缺氧/复氧组超微结构损伤严重,HPO组、EI1-EI3均可减轻H/R心肌细胞的损伤,但EI2组与EI1组和EI3组比较减轻更明显;F组无明显效果。结论缺氧/复氧对原代培养的成年大鼠心肌细胞可以造成明显损伤;EI后处理对原代培养成年大鼠心肌细胞缺氧/复氧损伤具有一定的保护作用。

Objective To observe the effects of EI postconditioning on myocardial ultrastructures of adult rats with hypoxia/reoxygenation. Methods Adult rat cardiomyocytes were separated and cultured,after the models with hypoxia and reoxygenation injury were established,cardiomyocytes were selected and randomly divided into 7 groups which were normal group（N）,hypoxia/reoxygenation group（H/R）,Hypoxia post-processing group（HPO）,the group of EI postconditioning with different concentrations（0.84 mmol/L,1.68 mmol/L and 2.52 mmol/L,EI1-EI3）and fat group（F）.To observe the myocardial cell growth characteristics and the change of ideology with inverted microscope.The ultrastructural changes of cardiomyocytes after hypoxia/reoxygenation injury was observed by use transmission electron microscope. Results Electron microscopy showed that the ultrastructure of normal group myocardial cell is normal,the ultrastructure of hypoxia/reoxygenation group was serious injuryed,group EI1-EI3 and group HPO can relieve the injury of H/R myocardial cell,But EI2 group reduced most obviously;group F had no obvious effect. Conclusion hypoxia/reoxygenation can lead significant damage to the original generation of myocardial cells in adult rats.EI has certain protective effect on the cardiomyocytes of adult rats with hypoxia/reoxygenation injury.