摘要
目的探讨14-3-3σ和Claudin-5蛋白在结直肠癌组织中的表达及临床意义。方法应用免疫组织化学染色方法检测14-3-3σ和Claudin-5在50例结直肠癌组织中的表达情况,结合其临床病理学资料,分析它们之间的相关性。结果14-3-3σ在对照组和肿瘤组中的阳性表达率分别为10%和58%,差异有统计学意义(P<0.001);Claudin-5在对照组和肿瘤组中的阳性表达率分别为82%和68%,差异无统计学意义(P>0.05);结直肠癌组织中14-3-3σ与Claudin-5表达无明显相关(P>0.05)。14-3-3σ表达与年龄、肿瘤大小、淋巴结转移(均P<0.05)有关,而与性别、分化程度、浆膜浸润无关(均P>0.05);Claudin-5表达与淋巴结转移有关(P<0.05),与性别、年龄、肿瘤大小、分化程度、浆膜浸润无关(均P>0.05)。结论14-3-3σ和Claudin-5蛋白在结直肠癌中表达明显增高,这两种蛋白均与结直肠癌淋巴结转移关系密切。
Objective To investigate the expressions of 14-3-3 σ and Claudin-5 and their clinicopathological characteristics in colorectal carcinoma（CRC）. Methods Pathological verified,50 cases of CRC were detected for the expressions of 14-3-3 σ and Claudin-5 by immuno-histochemical SP method. Results The positive expression rates of 14-3-3 σ were 10%,58% in normal mucosa and CRC tissue.Pairwise comparison showed There were statistical significantly different expression rates in different groups（P0.001）.The positive expression rates of Claudin-5 were 82%,68% in normal mucosa and CRC tissue.Pairwise comparison showed significantly different expression rates in different groups（P0.001）.The expression of 14-3-3 σ was associated with age,tumor diameter and LNM（P0.05）,but was not associated with sex,differentiation and serous membrane invasion（P0.05）.The expression of Claudin-5 was associated with LNM（P0.05）,but was not associated with sex,age,differentiation,tumor diameter and serous membrane invasion（P0.05）.There was no association between 14-3-3 σ and Claudin-5 in CRC. Conclusion The abnormally expressions of 14-3-3 σ and Claudin-5 are significantly associated with the incidence of LNM.
出处
《贵州医药》
CAS
2012年第10期865-868,共4页
Guizhou Medical Journal
