摘要
目的建立一种测定木瓜蛋白酶活力的共振散射(RS)新方法。方法在一定的条件下,丽春红S与酪蛋白形成的缔合微粒产生较强的共振散射信号和两个散射峰。基于木瓜蛋白酶催化酪蛋白水解,丽春红S与剩余酪蛋白结合,木瓜蛋白酶活力的增加,体系的共振散射强度(Ⅰ)降低。结果在pH=6.24的Sφrensen缓冲溶液、最强散射波长580nm处,酶活力在0.008～0.195USP/mL范围内,RS强度的降低(△I)与酶活力的增加有良好的线性关系,检出限为0.007USP/mL。结论建立了以丽春红S为RS探针的测定木瓜蛋白酶活力的新方法,操作简便、快速,灵敏度高,可用于实际样品中酶活力的测定。
Objective To establish a new resonance scattering （RS） method so as to determine the papain activity. Methods Under the invariable conditions, Ponceau S with Casein reacted to form association particles, which exhibited intensity resonance scattering （RS） signal and two scattering peaks. Based on the catalytic effect of papain on the hydrolysis of casein, Ponceau S be combined with the excess casein to form association particles. The scattering intensity （I） decreased with the activity of papain. Results Under pH： 6.24 S^0rensen buffer solution and at the strongest peaks 580 nm, there was a good linear relationship between the papain activity in the range of 0. 008-0. 195 USP - mL and the decreasing of the RS intensity decreasing. The detection limit was 0. 007 USP/mL. Conclusion The method with Ponceau S as RS probe is simple, quickly, sensitivity and be used to determine papain activity in the practical samples.
出处
《贵州医药》
CAS
2012年第11期973-975,共3页
Guizhou Medical Journal
基金
遵义市科技局资助[遵市科合字(2009)8号]
贵州省卫生厅资助[黔卫发(2011)140号]
贵州省科技厅资助[黔科合J字LKZ(2011)49号]
