甲状旁腺激素对去卵巢大鼠牙槽骨吸收的抑制作用

Parathyroid hormone (PTH) inhibits absorption of alveolar bone in ovariectomized rats

目的:观察甲状旁腺激素(human parathyroid hormone,PTH)对去卵巢大鼠牙槽骨的影响。方法:选用60只5月龄雌性大鼠随机分3组,其中两组作为药物治疗组和对照组均行全麻手术去除卵巢(ovariectomized,OVX),建立骨质疏松模型,另一组假手术组(sham-ovariectomized,sham-OVX)作为空白对照。6周后药物治疗组(PTH组)每日皮下注射人重组甲状旁腺激素(1-34)[recombinant human parathyroid hormone,rhPTH(1-34)],模型组(OVX组)和空白对照组(sham-OVX组)每日注射定量生理盐水,40 d后处死各组大鼠制取下颌骨标本。分析X线投照影像比较各组牙槽骨丧失程度;观察第一磨牙周围牙槽骨组织形态;检测牙槽骨破骨细胞数目进行比较。结果:影像学显示OVX组第一磨牙远中牙槽骨丧失(1.34±0.89)mm多于PTH组骨丧失量(0.78±0.62)mm和sham-OVX组骨丧失量(0.67±0.54)mm,差异具有统计学意义(P均<0.05);组织形态学染色显示OVX组牙槽骨骨质吸收较PTH组和sham-OVX组骨质吸收多;破骨细胞计数显示OVX组破骨细胞数(38.6±20.2)个分别多于PTH组(23.3±13.0)个和sham-OVX组(25.0±11.4)个,差异具有统计学意义(P均<0.01)。结论:PTH对骨质疏松大鼠牙槽骨的吸收有抑制作用。

Objective：To investigate the effect of human parathyroid hormone（PTH）on alveolar bone of ovariectomized（OVX） rats.Methods：Sixty female 5-month-old rats were divided into three groups randomly,two groups,which were ovariectomized and developed into osteoporotic models,were named as PTH treated group（PTH group） and the control group（OVX group）.Another group was sham ovariectomized（sham-OVX） as the blank control group（sham-OVX group）.Six weeks later,the PTH group was injected with recombinant human parathyroid hormone（1-34）[rhPTH（1-34）]once daily for forty days,while the other two groups were injected with saline.Then they were sacrificed and their mandibles were separated respectively for radiological imaging,histomorphological observation and osteoclastic cell couting.Results：The radiological imaging analysis showed that the loss of the distal alveolar bone of the first molar in OVX group was more than that of either PTH group or sham-OVX group [（1.34 ± 0.89）mm vs.（0.78 ± 0.62）mm,（0.67 ± 0.54）mm],both P 0.05.Meanwhile,the histomorphological observation suggested that the bone of OVX group was absorbed more than the other two groups.Besides,the cell counting results indicated that OVX group had more osteoclast than both PTH group and sham-OVX group（38.6 ± 20.2 vs.23.3 ± 13.0 and 25.0 ± 11.4 respectively,both P 0.01）,which showed significant statistical differences.Conclusion：The absorption of alveolar bone of the osteoporotic rats can be inhibited by PTH.