CEACAM6介导上皮间质转化增强胰腺癌细胞侵袭能力和耐药性的研究

CEACAM6 enhances the ability of invasion and chemo-resistance in pancreatic cancer cells by inducing epithelial-mesenchymal transition(EMT)

目的:研究CEACAM6对胰腺癌细胞株SW1990侵袭能力和耐药性的影响,并探讨上皮间质转化(EMT)在其中的作用。方法:筛选及鉴定过表达CEACAM6的稳定转染SW1990-CEACAM6细胞株,通过定量PCR和Western blot方法检测SW1990细胞株中EMT相关表型标志物的改变。通过Transwell实验检测过表达CEACAM6对SW1990细胞株侵袭的影响,MTT法检测耐药指数(RI)。结果:定量PCR及Western blot验证过表达CEACAM6细胞株SW1990-CEACAM6成功建立,其倾向于向间质细胞转化:波形蛋白(Vimentin)表达明显上调,E-钙黏蛋白(E-cadherin)表达下降。功能实验证实过表达CEACAM6具有促进SW1990胰腺癌细胞株侵袭作用,同时细胞株耐药性增强。结论:CEACAM6能够促进SW1990胰腺癌细胞株上皮间质转化,从而促进胰腺癌细胞的恶性转化。

Objective：To investigate the potential involvement of CEACAM6 in the invasion and chemo-resistance of pancreatic cancer cell SW1990 by inducing epithelial-mesenchymal transition（EMT）.Methods：After establishing and screening,CEACAM6overexpressing cell line SW1990-CEACAM6 was observed by inverted microscope,and the changes of EMT markers were determined by Q-PCR and western blot,the invasion ability was detected by transwell,the chemo-sensitivity to gemcitabine was determined by MTT assay.Results：Q-PCR and Western blot confirmed that SW1990-CEACAM6 cell line was established successfully.Overexpression of CEACAM6 made the pancreatic cell line SW1990 prone to mesenchymal phenotype,such as up-regulation of vimentin and down-regulation of E-cadherin.SW1990-CEACAM6 cell has greater activity of invasion and chemo-resistance to gemcitabine（P 0.05） than SW1990-EGFP cell.Conclusion：CEACAM6 could promote EMT and enhance malignant potential in terms of invasion and chemo-resistance in pancreatic cancer cell.