正交试验优选肾炎消白颗粒提取工艺及中试研究

Study on orthogonal test and medium enlarge test of extraction process of Shenyanxiaobai granules

目的:优选肾炎消白颗粒水提工艺。方法:采用正交试验法,以提取总固体得率和黄芪甲苷含量为指标,优选出肾炎消白颗粒的提取工艺,并进行中试研究。结果:实验室按处方量十分之一(132 g)投料,加水8倍量体积,提取3次,每次2 h;中试按处方量12倍(15.84 kg),工艺确定为加8倍量水煎煮3次,一煎、二煎各1.5 h,三煎1 h为最佳提取条件。结论:实验室得到的工艺与中试有差异。

Objective： To find out the optimum water extraction process for the preparation of Shenyanxi- aobai granules. Methods： During the study orthogonal test was used to find out the optimum extraction process of Shenyanxiaobai granules, with weights of total dissolved solids and Astragaloside IV content taken as targets, then medium enlarge test was studied. Results： The laboratory method determined the extraction process as follows： Eight times of water and extracting 2 h for 3 times, and the inventory rating was 132 g （ 1/10 recipe quantity）. Meanwhile, the optimum extraction process got by medium enlarge test was 8 times of water and extracting for 3 times. The first and second time extracted 1.5 h, and the last time extracted 1 h. The inventory rating was 15.84. Conclusion： There was difference between the extraction processes de- termined by laboratory study and medium enlarge test.