罗非鱼鳞明胶的制备及其酶解产物活性研究

Extraction of gelatin from tilapia scale and bioactivity of fish-scale collagen peptide

以罗非鱼鱼鳞为原料,通过热水提取鱼鳞中的明胶,利用多种蛋白酶对明胶进行酶解,测定其不同酶解产物的抗氧化性、血管紧张素I转换酶(ACE)抑制活性、α-葡萄糖苷酶(AG)抑制活性等生物活性。实验结果表明:100℃热水或120℃热水提取明胶1.5h的得率分别为20.2%和37.9%。6种蛋白酶(木瓜蛋白酶、胰蛋白酶、胃蛋白酶、酸性蛋白酶、中性蛋白酶、碱性蛋白酶)水解明胶后,胰蛋白酶水解液和碱性蛋白酶水解液的羟自由基清除力为76.7%和76%,高于Vc的65.6%;除中性蛋白酶对DPPH自由基没有清除力以外,其余5种蛋白酶均对DPPH自由基有清除力,但是低于Vc;胃蛋白酶水解液对α-葡萄糖苷酶的活性抑制作用最强,为67.1%,而中性蛋白酶、碱性蛋白酶和胰蛋白酶水解液对α-葡萄糖苷酶没有抑制作用。6种蛋白酶的水解产物均有的ACE抑制活性,没有抗凝血活性。

The gelatin was extracted from scale of tiliapia by hot water.Furthermore,the inhibitory activity of angiotensin I-converting enzyme（ACE）,the inhibitory activity of alpha-glucosidase（AG） and antioxidant activity of fish-scale collagen peptide were observed.The results showed that the yield of gelatin from hot water extraction at 100 ℃ for 1.5 h was 20.2%,and at 120 ℃ for 1.5 h the yield was 37.9%.The fish-scale collagen peptide was obtained by protease hydrolysis.The hydroxyl radical scavenging power activity of the trypsin hydrolyzate and alkaline protease hydrolyzate was 76.7% and 76%,higher than 65.6% of Vc.The DPPH scavenging activity was lower than Vc.The AG inhibition activity of pepsin hydrolysate was the highest,67.1%.While neutral protease,alkaline protease and trypsin gelatin hydrolyzates had no inhibitory effect on AG.The hydrolyzates of the 6 kinds of protease all showed ACE inhibitory effect and no anticoagulant activity.