摘要
以火腿肠为对象利用高效液相色谱法分析测定火腿肠中吲哚含量的方法。样品的去脂预处理采用固相萃取,脂肪用甲醇提取后,提取液通过Sep-pak C_(18)小柱,脂肪和脂肪酸被保留在C_(18)小柱上。色谱条件:流动相为乙腈:水=40:60;Eclipse XDB C_(18)、5μm、4.6×150 mm不锈钢柱,柱温29℃;荧光检测波长:EX 270 nm,EM350nm;吲哚测定方法回收率为97.3%。同时得出中吲哚的含量为1.44 mg/kg。
Taking ham sausage as the object of using high performance liquid chromatography determination of ham sausage fat extracted with methanol, extracting liquid by Sep-Pak C18 column, fat and fatty acids were retained in the C18 column. The chromatographic conditions were the mobile phase acetonitrile ： water--40 ： 60. The stainless steel column of Eclipse XDB C18, 5μm, 4.6× 150 mm, and the column temperature was 29 ℃. Fluorescence detection wavelength was EX 270 nm, EM 350 nm. Determination of indole recovery rate was 97.3%. At the same time that indole content was 1.44 mg/kg.
出处
《食品工业》
北大核心
2013年第2期188-191,共4页
The Food Industry
关键词
高效液相色谱法
肉制品
吲哚
检测
high performance liquid chromatography
meat products
indole
detection
