内置小室毛发重建模型

Hair reconstitution by the endo-chamber model

目的:建立一个高效、可重复的毛发重建模型,观察其诱导毛发的效果,探讨毛发发育的机制并为脱发的细胞学治疗提供实验基础。方法:制做假体小室置于裸鼠背部皮下,制备新生C57BL/6鼠真皮和表皮细胞混悬液,注射于裸鼠背部预置的小室内。7d后剪开表皮拆除小室,创面旷置,观察创面皮肤的愈合及毛发生长情况。结果:通过内置小室模型成功构建了毛发组织,新生毛发长出体表并具有良好方向性与生长密度,组织学检测其毛囊结构与正常组织无异。结论:内置小室是更为优化的毛发重建模型,成功诱导形成了正常结构的毛发组织,特别是毛发发育的早期,在有效避免外部因素的影响下,建立了类似于机体的内环境,更加有利于毛发的发育形成。

Objective The aims of this study were to build a efficient, reproducible hair reconstitufion model to evaluate the result of hair inducing and to explore the mechanism of hair morphogenesis and to provide an experimental basis for cell therapy of hair loss. Method An endo-chamber was made and grafted subcutaneously at dorsal site of the nude mouse. Cell suspension obtained from newborn C57BL/6 mice skin was injected into the endochamber. Seven days later, skin of the implanted site was cut off and the endo-chamber was removed. By leaving the wound untreated,the wound healing and hair reconstitution were observing every days after the endo-chamber removal. Result Hairs reconstituted successfully by the endo-chamber. New hairs grew with appropriate direction and density, which were proved no difference with the normal tissue by histological examination. Conclusion As an optimized hair reconstitution model, the endo-chamber assay which effectively avoided extraneous interference, built an internal environment suitable for hair formation and development, especially in the early period of hair morphogenesis.