HAART治疗对不同临床类型HIV-1感染者血清IL-18及IL-18BP水平的影响

The effect of HAART on levels of serum IL-18 and IL-18 BP in different clinical types patients with HIV-1

目的:探讨高效抗逆转录病毒治疗(HAART)对不同临床类型HIV-1感染者血清白细胞介素-18(Interleukin 18,IL-18)和白细胞介素-18结合蛋白(Interleukin 18 binding protein,IL-18BP)水平的影响。方法:酶联免疫吸附法(ELISA)检测67例经12个月以上HAART治疗有效的HIV-1感染者及18例健康人的血清IL-18和IL-18BP水平。采用流式细胞术测定HIV-1感染者血液中CD4+、CD8+T细胞水平。结果:不同临床类型HIV-1感染者血清IL-18、IL-18BP水平均高于正常对照组(P<0.05);A类HIV-1感染组CD4/CD8比值高于B类、C类HIV-1感染组(P<0.05,P=0.004,0.014)。CD4+T细胞<500的两组血清IL-18、IL-18BP水平均高于正常对照组(P<0.05);正常对照组血清IL-18和IL-18BP水平呈正相关(P<0.01,r=0.705)。结论:HAART治疗不能完全逆转HIV-1感染者免疫系统失衡,但艾滋病患者早期治疗可能降低机体免疫系统失衡的程度。

Objective To investigate the impact of HAART on the levels of IL-18 and IL-18BP in serum of different types HIV-I infected patients. Methods Serum samples obtained from 18 healthy subjects and 67 HIV-1 infected patients who responded well to HAART were analyzed by ELISA to determine their IL-18 and IL-18BP contents. The levels of CD4＋ T cell and CD8＋ T cell in blood of 67 HIV-1 infected patients were detected by flow cytometry. Results Compared with healthy persons, serum IL-18, IL-18BP increased in the serum of HIV-infected patients （P 〈 0.05）. The ratio of CD8 to CD4 of HIV-1 group A was higher than that of group B and C （P 〈 0.05, P = 0.004;P = 0.014）. Serum IL-18 and IL-18BP of HIV-1 groups which the levels of CD4＋ helper T cell was less than 200 were higher than the control group （P 〈 0.05 ）. A significant positive correlation was found between the levels of IL-18 and IL-18BP in healthy persons （P 〈 0.01 ,r = 0.705）. Conclusions HAART can＇t completely reverse the imbalance of HIV-1 infected patients＇ immune system. Early treatment may reduce the imbalance of immune systems.