RP-HPLC法同时测定十种五加属植物叶中三萜Chiisanoside和Chiisanogenin(英文)

Simultaneous Determination of Chiisanoside and Chiisanogenin from Leaves of Ten Kinds of Acanthopanax Miq.Plants by RP-HPLC

建立反相高效液相色谱法(RP-HPLC)测定chiisanoside和chiisanogenin的定量分析方法,利用最优色谱条件对十种中韩五加属植物叶中的三萜chiisanoside和chiisanogenin进行定量分析。研究结果表明,最佳色谱条件为:ODS-C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(55∶45);检测波长:205 nm;柱温:40℃;流速:1.0 mL/min。Chiisanoside和chiisanogenin线性范围和回归方程分别为16.5～181.5μg/mL,Y=5498356X-9738(r=0.9995)和4.88～78.0μg/mL,Y=5753131X-8289(r=0.9979),加样回收率分别为98.87%(标准偏差为1.14%)和98.83%(标准偏差为0.72%)。

A RP-HPLC method for simultaneous quantitative analysis of ehiisanoside and chiisanogenin was developed and optimized. Chiisanoside and chiisanogenin from leaves of ten kinds of Acanthopanax Miq. plants were quantitatively analyzed by the developed method. The best condition was established as follows ： the separation was performed with an ODS-C18eolumn （250 mm × 4.6 mm,5 μm） at 40 ℃ ,and the mobile phase was CH3CN-H2O （55： 45）. Chiisanoside and chiisanogenin were detected at 205 nm, the flow rate was 1 mL/min. The linear ranges of chiisanoside and chiisano- genin were 16.5-181.5 μg/mL and 4.88-78.0 μg/mL,and gave correlations （r） of 0.9995 （Y = 5498356X-9738） and 0. 9979 （ Y = 5753131X-8289）, respectively. The recoveries of ehiisanoside and chiisanogenin were 98. 87% （RSD was 1.14% ） and 98.83% （RSD was 0.72% ） ,respectively.