画眉草多糖提取及其保湿性能研究

Extraction and Moisture Retention of Polysaccharides from Eragrostis Teff

本文以脱脂画眉草为原料,用纤维素酶超声辅助法提取多糖;经脱蛋白、脱色、DEAE-纤维素分离纯化,得到3种多糖TPS1、TPS2、TPS3,提取率分别为0.42%、0.66%、0.54%。通过高效凝胶渗透色谱(HPGPC)分析,3种多糖的分子量分别为7886 Da、8467 Da、6366 Da;高效离子色谱(HPIC)测得TPS1组成单糖为果糖,TPS2、TPS3组成单糖为阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖、果糖、核糖和一种未知糖。对多糖TPS2的保湿和吸湿性研究结果表明TPS2的保湿和吸湿能力均优于常用保湿剂甘油、聚乙二醇400、1,3-丁二醇、壳聚糖。

The polysaecharides in the defatted eragrostis teff were extracted with cellulose enzyme and ultrasound auxilia- ry. The TPS1 ,TPS2 and TPS3 which were the main polysacchafides parts of eragrostis teff were obtained through purifi- cation on DEAE-cellulose, after the polysaccharides were taken off the protein with enzyme and decolored with activated carbon. Extraction ratios of the TPS1 ,TPS2 and TPS3 were 0.42% ,0.66% and 0.54%. The molecular weight of TPS1, TPS2 and TPS3 were 7886 Da,8467 Da and 6366 Da respectively measured by high-performance gel permeation chroma- tography. The components of the polysaccharides were detected by ion chromatography. The results showed that TPS1 contains fructose. Both TPS2 and TPS3 was composed of Ara, Glu, Gla, Xyl, Man, Fru, Rib and an unknown sugar. Mean- while the moisture retention and absorption performance of the TPS2 were studied. The results showed that the TPS2 moisture absorption and retention capacity are better than glycerol, polyethylene glycol 400 and chitosan.