摘要
以甜叶菊渣为原料,采用大孔树脂吸附和溶剂萃取法相结合的方法,得到90%以上纯度的总黄酮。通过对大孔树脂及溶剂萃取法的各影响因素进行研究,确定纯化甜叶菊渣中总黄酮的最佳工艺条件:AB-8型大孔树脂吸附流速为2 mL/min、上样液质量浓度1.5 mg/mL、上样液pH值为3.5、上样量4 BV,解吸液为50%乙醇溶液、解吸量5 BV、解吸流速为1.5 mL/min。优化后的甜叶菊总黄酮平均纯度为50.11%。后经乙酸乙酯在常温条件下萃取5次,得到甜叶菊渣中总黄酮纯度为91.8%。结果表明:通过AB-8型大孔吸附树脂和乙酸乙酯萃取相结合的方法,可以很好地纯化甜叶菊总黄酮。
Total flavonoids with purity higher than 90% were purified from Stevia rebaudiana by using the method of maeroporous resin adsorption and solvent extraction. The optimum conditions of maemporous resin adsorption were as fol- lows ：2 mL/min of sample adsorption velocity, 1.5 mg,/mL of sample concentration, pH 3.5,4 BV of sample loading a- mount ,5 BV of 50% ethanol as the eluent at a flow rate of 1.5 mL/min. The average purity of total flavonoids collected was 50.11% using the conditions above. The purity was increased to 91.8% after further extraction with ethyl acetate for five times in room temperature. The results showed that, AB-8 and ethyl acetate was suitable for the purification of to- tal flavonoids from S. rebaudiana.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第1期104-109,共6页
Natural Product Research and Development
关键词
甜叶菊
AB-8大孔树脂
溶剂萃取
总黄酮
纯化
Stevia rebaudiana
AB-8 macroporous resin
solvent extraction
total flavonoids
purification
