HPLC法同时测定舒眠胶囊中酸枣仁皂苷A和槲皮苷的含量

Simultaneous Determination of Berberine Jujuboside A and Quercitroside in Shumian Capsules by HPLC

目的:建立同时测定舒眠胶囊中酸枣仁皂苷A和槲皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Diamonsil C18反相柱,柱温为30℃,流动相为甲醇-乙腈-缓冲液(20:30:50,V/V/V),流速为1.5ml/min,检测波长为256nm,进样量为20μl。结果:酸枣仁皂苷A、槲皮苷检测质量浓度分别在10.168～101.68mg/L(r=0.9998)、9.285～92.85mg/L(r=0.9999)范围内与峰面积积分值呈良好的线性关系;平均加样回收率分别为99.7%、99.5%,RSD分别为1.98%、2.21%(n=6)。结论:该方法简便,结果准确,重复性及回收率均理想,可用于舒眠胶囊的质量控制。

OBJECTIVE： To establish a method for simultaneous determination of berberine jujuboside A and quercitroside in Shumian capsules. METHODS： HPLC method was adopted. A Diamonsil C18 reversed-phase column was used with mobile phase consisted of methanol-acetonitrile-buffer solution （20 ： 30 ： 50, V/V/V） at the flow rate of 1.5 ml/min. The detection wavelength was set at 256 nm and injection volume was 20μl. RESULTS： The linear ranges ofjujuboside A and quercitroside were 10.168-101.68 mg/L （r=0.999 8） and 9.285-92.85 mg/L（r=0.999 9） respectively. The average recoveries were 99.7% （RSD=1.98%, n=6） and 99.5% （RSD=2.21%, n=6）. CONCLUSION： The method is simple and accurate with sound reproducibility and recovery, which can be applied for the quality control of Shumian capsule.