海南核果木果实脂溶性成分的GC-MS分析

GC-MS Analysis of Liposoluble Constituents from Drypetes hainanensis Fruit

采用溶剂提取法、硅胶柱层析分离,获得海南核果木果实脂溶性提取物Ⅰ和Ⅱ,结合气相色谱-质谱联用对其化学成分进行分离、鉴定及分析。结果发现,海南核果木果实脂溶性提取物Ⅰ中主要为邻苯二甲酸二(2-乙基己基)酯(64.2%)和烷烃类(23.6%);从脂溶性提取物Ⅱ中鉴定出19种成分,主要为烯烃类(1.4%)、烷烃类(22.9%)、酯类(68.0%)以及植物醇(2.8%)等,酯类为饱和脂肪酸酯(35.8%),主要为软脂酸甲酯(29.0%)和十八碳酸甲酯(6.8%),不饱和脂肪酸酯(18.6%),主要为11,14-十八碳二烯酸甲酯(10.1%)和6,11-二十碳二烯酸甲酯(8.5%),另外,还含有邻苯二甲酸二(2-乙基己基)酯(13.6%)。

Liposoluble extracts Ⅰ and Ⅱ were isolated from Drypetes hainanensis fruit by solvent extraction method and silica column chromatography, and their chemical composition was identified and analyzed by GC - MS. The results show that the main constituents of the extract Ⅰ were bis （2- ethylhexyl） phthalate （ 64. 2% ）, alkanes （ 23.6% ）, etc. 19 compounds were found in the extract Ⅱ , mainly comprising olefin （ 1.4% ）, alkanes （ 22. 9% ）, esters （ 68.0% ）, phytol （ 2. 8% ）, etc. The esters mainly included saturated fatty acid ester（35.8% ） ,which was composed of hexadecanoic acid, methyl ester（29. 0% ） and octadecanoic acid methyl ester （6. 8% ）, unsaturated fatty acid ester （18.6%）, which was constituted of methyl 11,14-octadecadienoate（ 10. 1% ） and 6,11 -eicosadienoic acid methyl ester （ 8.5 % ）, and bis （ 2-ethylhexyl ） phthalate （ 13.6 % ）.