高效液相色谱法测定土壤中草除灵和高效氟吡甲禾灵的残留量

Determination of benazolin-ethyl and haloxyfop-R-methyl residue in soil by high performance liquid chromatography

建立了高效液相色谱法同时测定土壤中草除灵和高效氟吡甲禾灵残留量的方法。土壤样品用V(二氯甲烷)∶V(甲醇)=9∶1的混合溶剂提取,用配有紫外检测器的高效液相色谱仪测定,流动相为V(甲醇)∶V(水)=80∶20,紫外检测波长为225 nm。在0.02～1.00 mg/L范围内,检测草除灵和高效氟吡甲禾灵的色谱峰面积与其质量浓度呈良好的线性关系,相关系数分别为0.999 5和0.999 8。在添加水平为0.05～1 mg/kg时,二者的添加回收率均在85.1%～106.6%之间,相对标准偏差为5.2%～8.8%。草除灵和高效氟吡甲禾灵的仪器最小检出量分别为0.2和0.4 ng,方法定量限为0.05 mg/kg。

The analytical method of simultaneously determination of benazolin-ethyl and haloxyfop-R- methyl residue in soil was studied. Soil samples were extracted with methylene chloride-methanol mixture （9：1, WV）, and determined by high performance liquid chromatography （HPLC） with UV detector. Methanol-water （80：20, V/V） was used as mobile phase at a flow rate of 0.6 mL/min, and ultraviolet absorption wavelength was set at 225 nm for UV detector. Linear correlation of benazolin- ethyl and haloxyfop-R-methyl was 0.999 5 and 0.999 8 with concentration between 0.02 and i mg/L. When fortified from 0.05 to 1 mg/kg, the recoveries were 85.1% to 106.6%, with relative standard deriation（RSD） between 5.2% and 8.8%. The limit of detection of instrument for benazolin-ethyl and haloxyfop-R-methyl was 0.2 and 0.4 ng respectively, and the limit of quantification of the method was 0.05 mg/kg. The method can be used to analyze the residue of benazolin-ethyl and haloxyfop-R- methyl.