藏药仁青常觉的毒理学实验研究

Toxicological Experimental Study of Tibetan Medicine RQCJ

目的:观察藏药仁青常觉的毒理作用。方法:急性毒性实验:以一日最大耐受量测定小鼠灌胃仁青常觉的急性毒性实验。长期毒性试验:给大鼠灌胃给药3个不同剂量的仁青常觉,低剂量组(0.350g/kg)、中剂量组(0.700g/kg)和高剂量组(1.400g/kg),连续90天,空白对照组ig蒸馏水,观察大鼠一般状况、体重、摄食量,测定给药90天及停药15天血液生化指标、脏器系数、解剖学及病理组织。结果:测得小鼠最大耐受量大于30g/kg(此剂量相当于临床成人拟日服剂量的2142倍),未发现任何不良反应及死亡现象;各剂量组连续给药90天,未发现死亡,动物生长发育良好,外观正常,行为活动无异常,主要脏器系数、血液生化、血象等指标与空白对照组比较均无显著性差异。结论:通过实验,未发现明显毒性反应,证明临床剂量是安全的。

Objective. To investigate the toxicology of RQCJ. Methods ： Acute tocity test ： The maximum tolerated dose （MTD） of RQCJ were determined by intragastration administration in mice;Long-term toxic test： there doses of RQCJ0. 350g/kg, 0. 700g/ kg, 1. 400g/kg were given to rats by oral administration for 90 days, And control group were given distilled water instead . General conditions, body weight and food intake of rats were observed. The hematological and biochemistry parameters,organ coefficients and histomorphological figures were determined in 90 days of the experiment and in 15 days after the termination. Results： The maxi-mum tolerated dose （MTD） was 30g/kg（equivalent 441 times of the clinical dosage） , the rats did not appear dead and without any adverse reaction, the administration of 90d all the indexes mentioned above were normal in groups general conditions , hematological and biochemistry parameters, the main organ coefficient compared with the blank group is not significant differences. Conclusion：It is safe to be administrated in the dose prescribed by experiment.