Galectin-3及CyclinD1在良恶性甲状腺肿块中的鉴别价值

The differential diagnosis value of Galectin-3 and Cyclin D1 in benign and malignant thyroid mass

目的探讨Galectin-3和CyclinD1在良、恶性甲状腺肿块中的表达及鉴别价值。方法用免疫组化（SP）法检测31例结节性甲状腺肿（A组）、65例甲状腺乳头癌（B组）及10例正常甲状腺组织（c组）石蜡标本中Galectin-3蛋白和CyclinD1蛋白的表达，并结合临床资料分析。结果B组中Galectin．3的阳性率为83．03％（54／65），显著高于A组的19．35％（6／31）和C组（0％），前2组比较差异有统计学意义（X^2=36．360，P〈0．05）；B组中CyclinD1的阳性率为72．30％（47／65），显著高于A组12．90％（4／31）和C组（0％），前2组比较差异有统计学意义（X^2=29．740，P〈0．05）；联合检测Galectin-3和CyclinD1在良、恶性甲状腺肿瘤中的表达，发现阳性表达差异有统计学意义（P〈0．01），且Galectin-3和CyclinD1在B组中的表达也存在正相关（R=0．509，P〈0．05）。CyclinD1蛋白在有无淋巴结转移的甲状腺乳头癌中的阳性率分别为90％（18／20）和64．44％（29／45），差异有统计学意义（P〈0．05）。结论Galectin-3和CyclinD1在甲状腺乳头癌组织中的阳性表达存在正相关性，较结节性甲状腺肿和正常组织有强阳性表达，联合检测甲状腺组织中β-catenin及Galcetin-3的表达有助于甲状腺癌的诊断。

Objectives To investigate the expression and differential diagnosis value of Galectin-3 and Cyclin D1 in benign and malignant thyroid mass. Methods The expression of Galectin-3 and Cyclin D1 protein in 31 cases of nodular goiter（ group A）, 65 cases of papillary thyroid carcinoma（ PTC, group B）and 10 cases of normal thyroid tissues（ group C, the control group）was detected by SP immunohistochemical method. The clinical data were analyzed. Results The expression level of Galectin-3 in group B was 83.03% （54/65）, significantly higher than that in group A（ 19.35%, 6/31 ）and group C（0% ）. The difference of group A and group B in terms of the expression level of Galectin-3 protein had statistical significance（x^2 = 36. 360, P 〈 0. 05 ）. The expression level of Cyclin DI protein in group B was 72. 30% （47/65）, significantly higher than that in group A（ 12. 90%, 4/31 ）and group C（0% ）. The difference of group A and group B in terms of the expression level of Cyclin D1 protein had statistical significance （X2 = 29. 740, P 〈 0. 05）. The combination detection of the positive expression of Galectin-3 and Cyclin D1 in benign and malignant thyroid mass showed that the difference between group A and group B had statistical significance（ P 〈0. 01 ）. The expression of Galectin-3 and Cyclin D1 was positively corre- lated in group B （ R = 0. 509, P 〈 0.05 ）. The positive expression rate of Cyclin D1 protein was 90% （18/20） and 64. 44% （29/45）respectively in PTC patients with lymph node metastasis and without lymph node metastasis. The difference had statistical significance （P 〈 0. 05 ）. Conclusions The expression of Galectin-3 and Cyclin D1 is positively correlated in PTC and the expression of the 2 proteins in PTC is higher than that in nodular goiter and normal thyroid tissues, indicating that the 2 proteins can be used as valuable markers for patients with thyroid carcinoma. Combined detection of Cyclin D1 and Galectin-3 protein in thyroid tissues is useful for the diagnosis of thyroid cancer.