应用基因芯片分析植物内生菌醇提取物处理条件下水稻的基因表达图谱

Analysis on Gene Expression in Rice Under Endophytes Alcohol Extraction Treatment with Gene Microarray Technique

为探索植物内生菌R(人参)与D(越橘)醇提取混合物RD对水稻的促进生长、增强抗病性的作用机制,采用基因芯片技术对RD处理的水稻基因组进行分析,检测RD处理水稻后差异表达基因。结果显示,检测到差异表达基因1 171个,其中上调表达基因671个,下调表达基因500个。根据基因芯片的试验结果,采用Go注释系统对差异表达基因进行功能注释表明,主要为刺激应答、转录调控、生理功能、结合功能、细胞过程、代谢功能等。代谢途径的变化存在明显差异,表达量上调代谢通路39条,表达量下调代谢途径24条。说明植物内生菌R与D醇提取混合物处理影响水稻性状的表达不是某个基因孤立、单一的作用,而是多方面、多层次共同作用的结果。

In order to explore the promoting growth and disease resistance mechanism of endophytes alcohol extraction on rice, the gene expression in rice under the treatment of RD was analyzed by gene microarray technique. 1 !71 different expressed genes were identified in this study, including 671 up-regulated genes and 500 down-regulated genes. The explanation on the results of gene microarray through Go annotation system shponse to stimulus, transcription regulation, physiological process, cellular process and metabolic function. Significant difference was found among the pathway change, including 39 up-regnlated pathways and 24 down-regulated pathways, It can be concluded that the gene expression of rice under the treatment of endophytes alcohol extraction RD was ascribed to the co-effect of various aspects and multi level.