转基因玉米种子快速筛查方法研究与应用

Study and Application of a Rapid Screening Method for GM Maize Seeds

转基因种子快速筛查技术研究与应用对于加强农业转基因生物安全管理至关重要。将DNA热碱提取法和复合荧光PCR技术相结合,建立起玉米种子转基因成分快速筛查方法。结果显示,该方法不仅能保证检测的准确性和灵敏性,而且还大大降低假阳性发生率,提高转基因筛查的效率,适合大批量样品的快速检测。

A rapid and high efficient screening method of GM crop seeds is the important technical support for strengthening regulation of the agricultural GMO. In this paper, a rapid screening method of GM maize seeds was established by integrating the DNA extraction method of hot alkaline and the multiplex real-time fluorescent PCR technology. The results showed that the rapid screening method can not only ensure the detection accuracy and sensitivity, but also greatly reducing false-positive incidence and improve the efficiency of the GMO screening, and which is suitable for the rapid detection of large numbers of samples.