复合EST-SSR标记在大白菜品种鉴定中的应用

Cultivars Identification of Chinese Cabbage Using Multiplex EST-SSR Markers

利用EST-SSR分子标记技术,对12份大白菜品种鉴定方法进行了研究。通过对GenBank数据库中公布的大白菜EST序列的分析,合成了30对核心EST-SSR引物,其中21对均可得到有效扩增,10对具有较高的多态性。为了更加高效的对大白菜品种进行鉴定,根据这10对多态性较高的引物对12份市售大白菜品种鉴定的结果及扩增条带的相互位置差异,研制了由多个引物对组成的2套复合EST-SSR标记,建立了多重SSR的反应体系及反应条件。筛选得到的2套标记组合,多态率分别为88.9%和97.0%,多态信息量均为0.910%,数据表明这2套复合标记技术可以表达大白菜品种间的多态性,高效准确的对12份大白菜品种进行鉴定。

In this research, 12 Chinese cabbage cultivars identification method was studied by EST-SSR molecular markers technique. The results showed that there were 21 pairs of EST-SSR primers showing the amplification and 21 pairs of EST-SSR primers showing the high polymorphism among 30 pairs of screened SSR primers, which were designed from expressed sequence tags （ ESTs ） in GenBank. In order to identify Chinese cabbage high performance, using the identification results and the mutual position difference bands of 10 pairs of highly polymorphic primers on 12 Chinese cabbage varieties cultivars, make up 2 sets of multiplex EST-SSR markers. The polymorphic rate were 88.9% and 97.0%, polymorphism information content was 0.910%, and results indicate that the 2 sets of multiplex EST-SSR markers could be used to indentification 12 Chinese cabbage cultivars high performance.