原生质体融合技术选育分解草酸乳酸菌菌株的研究

Screening of the Oxalate-degrading Lactobacillus Strains Using Protolast Fusion Technology

将具有草酸分解能力的乳双歧杆菌和具有耐氧特性的嗜酸乳杆菌进行原生质体融合,其最佳条件为:50%的PEG6000,融合温度30℃,融合时间为7 min,CaCl2浓度为0.02 mol/L,MgCl2浓度为0.5 mol/L,在此条件下融合率可达7.6%。从中筛选出同时具有耐氧特性和草酸分解能力的融合子,草酸分解率为13.4%。

Bifidobacterium lactis with oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and can be used to prevent and treat kidney stone diseases. While Bifidobacterium lactis is poorly oxygen-tolerant, that can not be employed as microbial ecological agents. In this study, protoplast fusion was studied between Bifidobacterium lactis with oxalate-degrading capacity and oxygen,tolerant lactobacillus acidophilus. Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, fusion time 7 rain, fusion temperature 30℃, concentration of CaCl2 0.02 mol/L and the concentration of MgCl2 0.5 mol/L, the fusion rate reached 7.6%, and a oxygen-tolerant fusant showing the level of oxalate degradation at 13.4% was obtained.