HPLC法同时测定不同产地决明子中12种蒽醌类成分的含量

Simultaneous Determination of Twelve Anthraquinones in Cassiae Semen from Different Areas by HPLC

目的建立决明子中12种蒽醌类成分的含量测定方法。方法采用HPLC法,色谱柱:Diamonsil C18(150mm×4.6 mm,5μm),流动相:乙腈-0.1%甲酸水溶液梯度洗脱;流速:1.0 ml.min-1;检测波长:280 nm,柱温:25℃。结果决明子苷、2-葡萄糖基橙黄决明素、2-葡萄糖基决明素、2-葡萄糖黄决明素、橙黄决明素、大黄酸、黄决明素、决明素、芦荟大黄素、1-去甲基决明素、大黄酚、大黄素甲醚完全分离;峰面积与其浓度呈良好的线性关系;平均回收率分别为98.1%(RSD1.2%)、98.9%(RSD2.1%)、96.9%(RSD2.9%)、97.1%(RSD2.1%)、102.8%(RSD1.3%)、98.9%(RSD2.0%)、96.2%(RSD1.7%)、98.5%(RSD1.9%)、103.2%(RSD1.9%)、102.3%(RSD2.6%)、98.9%(RSD1.6%)、97.67%(RSD1.4%)(n=6)。结论该法灵敏、准确、重复性强,可用于决明子中蒽醌类成分的含量测定。

Objective To establish a method for determining twelve anthraquinones in cassia semen. Methods Diamonsil Cls （150 mm x4.6 mm,5 μm）was used with the mobile phase consisting of acetonitrile-0.1% formic acid solution gradient at a flow rate of 1.0 ml .min -1. The detection wavelength was set at 280 nm and the column temperature was 25 ℃. Results Complete separation was obtained within 80 min for twelve compounds （ cas- side, 2-gluco-aurantioobtusin, 2-gluco-obtusin, 2-gluco-chrysoobtusin, auranto-obtusin, rhein, chrysoobtusin, obtusin, aloe-emodin, 1-desmethylobtusin, chrysophanol, physcion）. A linear relationship between the peak area and concentration of each compound was obtained. The average recovery（ n = 6） of the above compounds was 98.1% （RSD1.2%） ,98.9% （RSD2. 1%） ,96.87% （RSD2.87%） ,97. 12% （RSD2. 1%）, 102.8% （RSD1.3%） 98.92% （RSD2.0%）,96. 15% （RSD1.7%）,98. 5% （RSD1.9%）, 103. 2% （RSD1.9%）, 102.3% （ RSD 2.6% ） ,98.9% （ RSD1.6% ） ,97.67% （ RSD1.4% ）. Conclusion The method established, sensitive, accurate and well repeatable, can be used for determination of anthraquinones in cassia semen.