过氧化物酶用于山楂嫁接亲和性早期鉴定研究

Early Detection of Graft-compatibility in Crataegus L.Using POD Analysis

木本植物嫁接体生长多年才表现出不亲和,为实现猩红英国山楂不同嫁接组合砧穗亲和性的早期鉴定,研究了猩红英国山楂及其砧木过氧化物酶(POD)的同工酶谱带,并对嫁接后各组合砧木和接穗的POD活性进行了动态测定。结果表明,砧穗POD同工酶谱带相同值的大小可以反映嫁接亲和性的强弱。嫁接后,砧穗POD活性差异小且逐渐变小的猩红英国山楂/单子山楂组合亲和性强,砧穗POD活性差异大且逐渐增大的猩红英国山楂/辽宁山楂组合亲和性差。因而认为,砧穗的POD活性可以作为山楂嫁接亲和性的早期鉴定指标。

Experiments were conducted to analyze the peroxidase isoenzymes of the ornamental hawthorn cultivar（Crataegus laevigata ＇Paul＇s Scarlet＇） with three kinds of hawthorn rootstocks（C.pinatifida,C.sanguinea,C.monogyna）,and to measure the POD activity of scion and rootstock bark after grafting.The results were as follows：the same count value of POD isoenzyme of scion and rootstock was related to graft-compatibility,the former was bigger and the latter was stronger.As growth of the graft union,the difference value of POD activity between rootstock and scion became smaller from Clps/Cm of which the compatibility was stonger,and from Clps/Cs,the difference value became bigger,while the compatibility was weaker.So the POD activity at graft unions could be used as a method for early detection of graft-compatibility.