Co^(60)-γ射线诱变宝巾(Bougainvillea)的生物学效应研究

Study on the Biological Effect of Bougainvillea Induced by Co^(60)-γ Ray

研究宝巾品种辐射处理后VM1代的生物学效应,为宝巾的辐射育种提供理论依据。以宝巾(B.glabra)和毛宝巾(B.spectabilis)为材料,通过不同剂量水平的Co60-γ射线处理,研究辐射剂量与其存活率、变异率的相关性,观察记录不同剂量下诱变株的生长表现,总结主要的变异性状类型。结果表明:在一定剂量Co60-γ射线范围内,辐射剂量和宝巾品种存活率呈负相关,和变异率呈正相关;毛宝巾的剂量敏感区域在50～150Gy,而宝巾的剂量敏感区域可能在150～200Gy,毛宝巾的辐射敏感性要高于宝巾;建立了毛宝巾剂量与存活率的线性回归方程:y=-0.409 8x+85.176,R2=0.989 8,并由此计算毛宝巾的半致死剂量(LD50)为86Gy;VM1代出现的各类叶变异性状主要为叶卷曲、叶残缺、叶皱缩、黄化叶等。

This paper studied the biological effect in VM1 generation of Bougainvillea varieties treated by Co^60-γ ray and provided the theoretical reference for Bougainvillea irradiation bree-ding.The correlations of the dose and survival was studied,as well as variation rates by different dosage levels of Co^60-γ ray with B.glabra and B.spectabilis.The growth performance for thevariants was observed and recorded,and then the variation character types were generalized.Theresults showed that there is a negative correlation between dose and survival,but a positive between dose and variation rates.The radio sensitivity for B.spectabilis is superior to B.glabra.The regression equations were built with dose and survival for B.spectabilis：y=-0.409 8x＋85.176,R2=0.989 8,calculating the half a lethal dose：86 Gy.In VM1 the leaf variations mainly were characterized by curly leaf,leaf deformity and leaf shrinkage,yellowing leaf,etc.