实时荧光定量PCR法测定福州地区高危型HPV感染型别与病毒载量

Detecting the genotype and viral load of human papillomavirus in Fuzhou with real-time fluorescence quantitative PCR

目的利用实时荧光定量PCR技术进行HPV-DNA分型定量检测,观察HPV亚型感染的分布情况,初步探讨HPV感染主要基因型别及病毒载量与宫颈病变的关系。方法随机收集本院妇科门诊患者108例,建立病历档案,采集患者宫颈脱落细胞样本,进行HPV-DNA分型定量检测。结果108例门诊患者检出HPV阳性14例,阳性率为12.96%,其中单纯HPV次要高危阳性感染9例,占64.28%;单纯HPV-16阳性感染2例,单纯HPV-31阳性感染1例;HPV-16、HPV-31和HPV次高危型三重感染1例,HPV-16和HPV-18/45的二重感染1例。结论本组患者HPV次高危型感染亚型几率最大,HPV高危型和次要高危型的感染与宫颈病变有高度的相关性,且病毒的感染量与病变的程度有一定的相关性,同时也显示HPV实时荧光定量PCR技术是一种有效的HPV检测手段。

Objective To study the relationship among HPV infection,HPV main genotype and cervical lesions.In the same time,to preliminary survey the distribution of HPV main genotype in the positive cases.Methods The cervical exfoliated cells from gynecological clinic patients were obtained.The sample＇s HPV-DNA were detected by using real-time quantitative fluorescence PCR（RT-FQ-PCR） technique.Results There were fourteen positive cases in the 108 clinic patients cases,total positive rate was 12.96%,Including nine positive cases of HPV secondary high-risk type unmixed,two positive cases of HPV-16 unmixed,one positive cases of HPV-31 unmixed,one positive cases of HPV16,31,and secondary high-risk type mixed,and one positive cases of HPV16,18/45 mixed.Conclusion The secondary high-risk genotype of HPV has the highest rate in the HPV infection among all those subtypes.The cervical lesions have high relationship with 16,18/45,31 and secondary high-risk genotype of the human papillomavirus.RT-FQ-PCR detection of HPV is an effective technique.