松树皮提取物原花青素对心肌缺血再灌注损伤大鼠的保护作用

Effects of pine bark extract procyanidins on myocardial ischemia reperfusion injured rats

目的探讨松树皮提取物原花青素对心肌缺血再灌注损伤(MIRI)大鼠的保护作用,为进一步研究此药对缺血性心血管疾病的防治提供依据。方法用60只雄性SD大鼠随机分成6组,即假手术组、模型组、复方丹参对照组、松树皮提取物原花青素低剂量组(60 mg.kg-1)、中剂量组(80 mg.kg-1)和高剂量组(100 mg.kg-1)。将麻醉大鼠冠脉结扎30 min后,再灌注120min造成心肌损伤模型。均与结扎LAD前20 min给予药物和生理盐水。结果松树皮提取物原花青素能降低缺血再灌注后血清内皮素(ET-1)浓度,而且能够升高一氧化氮(NO)的水平以及提高超氧化物歧化酶(SOD)活性。结论松树皮提取物原花青素对心肌缺血再灌注损伤的大鼠具有保护作用。

Objective To explore the effects of pine bark extract procyanidins on myocardial ischemia-reperfusion injury in rat,and to provide basis for drug prevention and treatment of ischemic cardiovascular disease.Methods 60 male SD rats were randomly divided into 6 groups,namely compound danshen group,sham operation group,ischemia-reperfusion group,low-dose group（60 mg·kg-1）,middle-dose group（80 mg·kg-1） and high-dose group（100 mg·kg-1）.Myocardial injury model was made by ligating the coronary artery for 30 min followed by reperfusion 120 min in anesthetized rat.All animals were given the medicine or normal saline before the experiment.Results Pine bark extract procyanidins can decrease the density of ET-1 after reperfusion,increasing the content of NO and the activities of superoxide dismutase（SOD） activity.Conclusion Pine bark extract procyanidin has protective effect on myocardial ischemia-reperfusion injury in rats.