ARHⅠ和DAPK在人脑胶质瘤组织中的表达及意义

Expression and clinical significance of ARHⅠ and DAPK in Human Glioma

目的:探讨ras同源家族成员Ⅰ(aplysia ras homologⅠ,ARHⅠ)基因和死亡相关蛋白激酶(death associatedprotein kinase,DAPK)基因在正常脑组织及脑胶质瘤组织中的表达及临床意义。方法:采用免疫组织化学SP染色技术测定10例正常脑组织及47例脑胶质瘤组织中ARHⅠ和DAPK的表达。结果:ARHⅠ和DAPK蛋白在正常脑组织中的阳性表达率最高,并且随着胶质瘤恶性程度的增高,ARHⅠ和DAPK的阳性细胞表达率逐渐下降,其在高级别胶质瘤中的表达明显高于低级别胶质瘤,差异有统计学意义(P<0.05),在胶质瘤中ARHⅠ和DAPK蛋白表达呈显著正相关(r=0.723,P<0.05)。结论:ARHⅠ和DAPK蛋白在脑胶质瘤中的表达与肿瘤的临床病理分级密切相关,ARHⅠ和DAPK表达下调可能与脑胶质瘤的发生发展有关。

Objective ： To investigate the expressions of aplysia ras homolog I （ ARH I ） and death as- sociated protein kinase （DAPK） in normal brain tissues and glioma tissues and to analyse their clinical path- ological significance. Methods： The SP immunohistochemical method was used to detect the expressions of ARH I ans DAPK in 10 case of normal brain tissues and 47 case of human glioma samples. Results ： The positive of ARH I and DAPK were the highest in normal brain tissues. In glioma samples of different grades, the expression levels of ARH I and DAPK decreased significantly with the increase of tumor malig- nancy. There was significant difference in the positive expression of ARH I and DAPK between high grade and low grade glioma tissues. The difference was significant （P 〈 0.05 ）. The expression of ARH I was cor- related positively with the expression level of DAPK（ r = 0.723 ,P 〈 0.05 ）. Conclusion： The expression of ARH I and DAPK were closely correlated with the ARH I and DAPK was involved in the initiation and clinical pathological grades. The down-regulation of progression of glioma.