CD123在儿童急性B淋巴细胞白血病中的表达及其在微小残留病检测中的应用

Expressions of CD123 in childhood B-lineage acute lymphoblastic leukemia and the application of CD123 in minimal residual disease detection

目的:检测细胞表面抗原CD123在儿童急性B淋巴细胞白血病(B-lineage acute lymphoblastic leukemia,B-ALL)中的表达,并探讨其在儿童B-ALL微小残留病(minimal residual disease,MRD)检测中的应用及意义。方法:以健康儿童骨髓淋巴细胞为对照,应用多参数流式细胞术检测91例儿童B-ALL患者骨髓淋巴细胞免疫表型及CD123的表达,其中78例B-ALL患者进行了骨髓细胞染色体培养及细胞遗传学分析。用CD10/CD123/CD34/CD19等抗体组合对其中CD123阳性表达的65例B-ALL患者进行MRD标记的筛选和监测。结果:健康儿童骨髓淋巴细胞CD123为阴性表达,91例儿童B-ALL患者中65例骨髓淋巴细胞CD123阳性表达(阳性率71.43%),且表达水平与白血病细胞成熟度呈负相关,CD34高表达患儿CD123表达水平高于CD34低表达患儿;细胞遗传学分析超二倍体组患儿CD123表达水平高于非超二倍体组;筛选到47例(占51.65%)B-ALL患儿CD123高表达。结论:儿童B-ALL患者多数表达CD123,表达水平与细胞成熟度呈负相关,CD123可用作B-ALL患儿的MRD监测标记。

Objective： Detecting the expression of CD123 in childhood B-lineage acute lymphoblastic leukemia （B-ALL） and discussing the application and significance of CD123 in minimal residual disease （MRD） detection. Methods： With the normal children bone marrow lymphocytes as control, multiparame- ter flow cytometry was used to study the bone marrow lymphocytes immunophenotype and the expression of CD123 in 91 cases of children with B-ALL, 78 cases of which were underwent bone marrow cells chromo- some cultivation and cytogenetic analysis. 65 cases of B-ALL children with CD123 positive expression were screened and monitored by CDlO/CD123/CD34/CD19 in MRD detection. Results： The expression of CD123 in normal children bone marrow lymphocytes was negative, while 65 cases of 91 in B-ALL children was positive（positive rate 71.43% ）, and the expression level of CD123 was negatively correlated with the leukemic cells maturity. When children had a high expression of CD34, the expression of CD123 was also in a high level. The results of cytogenetic analysis showed that hyperdiploid B-ALL children had a higher level of CD123 expression, when comparing with the non-hyperdiploid cases. Forty-seven cases of B-ALL children （51.65%） had a high level of CD123 expression, and CD123 could be used as an effective mark- er for MRD monitoring. Conclusion： Most of B-ALL children had an expression of CD123, which was neg- atively correlated with the leukemic cells maturity. CD123 can be used as a maker for MRD monitoring in childhood B-ALL.