松乳菇多糖抗肿瘤和免疫调节活性研究

Anti-tumor and Immunomodulatory Activity of Polysaccharide from Lactarius deliciosus Fruit Bodies

为探讨松乳菇(Lactarius deliciosus)多糖纯品的抗肿瘤活性和免疫调节活性,采用腹腔注射给药测定昆明种小鼠抑瘤率和脏器指数,采用MTT法测定肿瘤细胞生长抑制率及肝细胞增殖率,用倒置显微镜观察肿瘤细胞的生长情况。结果表明:在体内,松乳菇多糖能够显著抑制移植性肿瘤S180的生长,并呈一定的剂量依赖关系;14和28mg/kg.d的抑瘤率分别为48.71%和68.51%,且对小鼠脏器均无明显的损伤和毒副作用。在体外,松乳菇多糖可促进免疫细胞增殖,其对人正常肝细胞增殖的促进能力与浓度呈正相关。在一定范围内,松乳菇多糖可抑制肿瘤细胞增殖,并与剂量成正比,且作用于不同的肿瘤细胞有不同的效果。松乳菇多糖在高剂量时对人喉癌Hep-2的抑制作用较好,给药浓度为600μg/mL时,其最高抑制率达50.8%;而松乳菇多糖在低剂量时对人肝癌HepG-2有一定的抑制作用,给药浓度为150μg/mL时,其最高抑制率达32.76%。

The antitumor and immunomodulatory activity of a polysaccharide （LDG-A） extracted from Lactarius deliciosus fruit bodies were evaluated. Polysaccharide was administered by intraperitoneal injection into tumor-bearing Kunming mice and the tumor inhibition rate and various organ indices were determined. LDG-A significantly inhibited the growth of S180 tumor in vivo, with inhibition rates of 48.7% and 68.5% recorded at dose levels of 14 mg/kg/d and 28 mg/kg/d, respectively. No obvious injury to internal organs or other side effects were evident. LDG-A also promoted the in vitro proliferation of normal liver cells and inhibited the in vitro proliferation of Hep-2 and HepG-2 tumor cells in a dose-dependent manner. Hep-2 cell proliferation was inhibited 50.8%, and HepG-2 cell proliferation 32. 7%, at LDG-A concentrations of 600 μg/mL and 150μg/mL, respectively.