摘要
为了满足当前对于食品安全实时监控的需要,对腹泻性贝类毒素DSP的两种快速筛选方法———蛋白磷酸酶抑制法和酶联免疫法开展了研究,首先根据国内外相关标准的规定制定了这两种方法的筛选检出限,再对标准溶液及模拟阳性样品进行了检测,并进行了重复性验证,最后分析了这两种方法的检测原理并进行了优势比较。检测结果显示这两种筛选方法均具有准确率高,重复性较好的优点,本文提出的筛选检出限(200μg/kg)远低于我国相关标准的规定(600μg/kg),都不失为检测腹泻性贝类毒素的理想筛选方法。
Abstract：To fulfill the needs for real-time monitoring of food safety, we investigated the two screening detecting method of DSP： protein phosphatase inhibition assay and ELISA assay. The threshold value for detection limit was determined at first. Then, the standard solution, the artificially contaminated samples were explored use both methods. The reproducibility of this method was also discussed. As a result, the method of these two assays both showed high veracity and good reproducibility. The detection limit we reached （200 μg/kg） was much lower than the set standard of relevant stipulate （600 μg/kg）. Therefore, both method can be used as an ideal way to detect DSP in aquatic products.
出处
《食品研究与开发》
CAS
北大核心
2012年第12期127-130,共4页
Food Research and Development
