胃癌中p53蛋白K370位点甲基化在新辅助化疗的作用和临床意义

Neo-adjuvant chemotherapy for gastric cancer caused by methylation at the K370 site of p53 protein

目的研究胃癌患者外周核细胞p53蛋白K370位点甲基化在新辅助化疗的判断的作用和临床意义。方法应用甲基化特异性聚合酶链反应(MSP)方法分别检测胃癌组织及外周血有核细胞p53蛋白K370位点甲基化和化疗疗效以及病理分期的相关性,并通过Pearson统计学方法分析其相关性。结果 110例胃癌患者中,K370的甲基化在胃癌组织及全血中的表达具有正相关性,K370的甲基化率随着病理分期增高而增高,胃癌外周血中p53蛋白K370甲基化表达情况与病理分期密切相关;胃癌患者经过新辅助化疗治疗后,甲基化平均敏感性阴性组为60.6%,阳性组为25.3%,甲基化阴性组的敏感性高于阳性组,差异有统计学意义(P<0.05)。结论胃癌全血标本中p53蛋白K370位点甲基化状态有望作为胃癌新辅助化疗方案敏感性的依据。

Objective To explore the correlation between methylation status at the K370 site of p53 protein in peripheral blood karyocyte with effects of neo-adjuvant chemotherapy （NACT） for gastric cancer. Methods MSP was used to detect the expression of methylation at the K370 site of p53 protein from gastric cancer tissue and peripheral blood karyocytes, and the relationship between NACT effects of gastric cancer and methylation status was analyzed with the Pearson’s correlation coefficient method. Results In 110 patients with gastric cancer, the K370 methylation rate increased along with the pathological staging, showing a positive correlation. There was no significant difference in methylation rate in gastric cancer tissue and peripheral blood （P0.05）. Methylation at the K370 position of p53 protein was closely related to pathological staging in gastric cancer tissue and peripheral blood. The effect of NACT in the non-methylation group was 60.6% and in the methylation group 25.3%. There was a significant difference in therapeutic effects of NACT （P0.05）. Conclusion In the peripheral blood of gastric cancer, methylation status at the K370 position of p53 protein is closely related to the therapeutic effects of NACT.