蛇含委陵菜提取物对人工感染鸡白痢的治疗效果

Therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken

【目的】探讨蛇含委陵菜提取物对雏鸡白痢的治疗效果。【方法】采用不同提取方法(水煎法、水煎醇提法、微波提取法、乙酸乙酯提取法、乙醇分级提取法)对蛇含委陵菜进行提取,测定不同极性段提取物的最小抑菌浓度(MIC),筛选出最佳提取方法,然后以蛇含委陵菜水提物自拟高、中、低3个剂量组,经口灌服人工感染鸡白痢沙门氏菌的1日龄雏鸡,以诺氟沙星为药物对照,评价其对雏鸡白痢病的治疗效果。【结果】水煎法获得的蛇含委陵菜水提物对鸡白痢沙门氏菌和鸡大肠杆菌的抑制效果最佳,MIC分别为5.21和10.41mg/mL;在人工感染治疗试验中,雏鸡在感染鸡白痢沙门氏菌18h后,以每天经口灌服1次蛇含委陵菜水提物(0.75mL/羽),连续3d,其治疗有效率达86.7%。【结论】蛇含委陵菜水提物对革兰氏阴性菌具有良好的抑制效果,安全低毒,原材料价廉易得,具有较高的研发价值。

[Objective]The present experiment was aimed to evaluate the therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken.[Method]Five extracting methods viz.,water decoction,water decoction combined with ethanol extracting,microwave extracting,ethyl acetate extracting and ethanol grading extracting were used to extract Potentilla kleiniana and minimal inhibitory concentration（MIC） in several extracts of different polarity zones were measured to screen out the best extraction method;subsequently,the Potentilla kleiniana extract with three different dosages（high,moderate,and low） were drenched to treat one-day-old chicks infected with artificial Salmonella pullorum,then the therapeutic effects were assessed using norfloxacin as the medical control.[Result]The water extract,obtained from water decoction,showed the most potent antimicrobial activity to Salmonella pullorum and Escherichia coli in chicken,and the MIC of Salmonella pullorum and Escherichia coli were 5.21 and 10.41 mg/mL,respectively.The optimal treatment effective rate of chicken reached 86.7% through drenching 0.75 mL/chicken once a day for 3 days consecutively after infecting Salmonella pullorum for 18 hours.[Conclusion]Safe,inexpensive,and low-toxic Potentilla kleiniana extract could effectively inhibit gram-negative bacterium;hence,Potentilla kleiniana extract is of high research and developmental value.