太旨黄特型绍兴黄酒对骨质疏松症大鼠的影响

Effect of Taizhihuang Shaoxing Special Rice Wine on Rats with Osteoporosis

目的:观察探讨太旨黄特型绍兴黄酒对骨质疏松症大鼠的影响及其作用机制。方法:大鼠维甲酸灌胃建骨质疏松模型,70只健康雌性Wistar大鼠随机分为空白对照组、模型对照组、普通黄酒对照组、太旨黄纯药物组、太旨黄低剂量组、太旨黄中剂量组和太旨黄高剂量组7组,每组10只。分别给予相应药物,每天1次,连续灌胃90 d。酶比色法检测血清碱性磷酸酶(ALP),抗酒石酸酸性磷酸酶(StrACP);检测大鼠双侧股骨骨密度(BMD),骨矿含量(BMC)。HE染色,观察骨组织病理形态学。结果:与普通黄酒组比较,太旨黄各组有显著性差异(P<0.05)。结论:太旨黄特型绍兴黄酒对骨质疏松症有一定的治疗作用。

Objective：To observe the effect of Taizhihuang Shaoxing special rice wine on rats with osteoporosis and study the mechanism of action. Methods： Rats model of osteoporosis was induced by retinoic acid. 70 healthy female Wistar rats were randomly divided into seven groups with 10 rats in each： blank control group,model control group, ordi- nary rice wine control group, Taizhihuang pure drug group, low, middle and high dose of Taizhihuang groups. The rats were treated with different medicine according to their weights, once a day. After 90 days＇treatment, serum alkaline phos- phatase（ALP） and tartrate- resistant acid phosphatase （StrACP）were measured by enzyme colorimetric method. The hi- lateral femoral rats bone mineral density（BMD） and bone mineral content（BMC） were detected. Bone tissue pathological morphology was detected by hematoxylin - eosin （ H - E ） staining. Results ： Compared with ordinary rice wine control group, each group of Taizhihuang was significantly different （ P 〈 0.05 ）. Conclusion ： Taizhihuang Shaoxing special rice wine has a therapeutic effect on osteoporosis.