补肾活血方对前列腺增生模型大鼠膀胱胶原沉积的影响

Effect of Bushen Huoxue Recipe on Bladder Collagen Deposition Prostate Hyperplasia in Rats

目的:观察补肾活血方补肾活血方对前列腺增生大鼠膀胱组织胶原沉积的影响。方法:清洁级雄性Wistar大鼠48只,随机分为正常组、模型组、模型加补肾活血方组、模型加坦索罗辛组4组。除正常组外,其它实验大鼠以去势加睾酮注射法复制大鼠BPH模型。于去势第一天起,各组大鼠分别给予相应药物灌胃。模型+补肾活血方按1 mL/100(g.d)体重予补肾活血方(由淫羊藿、枸杞子、穿山甲、丹参、菟丝子等组成,含生药量2.34g/mL)灌胃,模型加坦索罗辛组按1.8μg/100(g.d)体重予坦索罗辛灌胃。其余各组予等体积生理盐水灌胃。均每日1次,连续38 d后检测各组大鼠前列腺、膀胱湿重及脏器指数,免疫组织化学法观察膀胱组织内胶原沉积。结果:模型组膀胱胶原Ⅲ阳性面积率、总IOD等均明显高于空白组(P<0.01)。补肾活血方、坦索罗辛组膀胱胶原Ⅲ阳性面积率及总IOD均明显较模型组低(P<0.01)。膀胱胶原Ⅰ沉积情况与胶原Ⅲ类似。模型组膀胱胶原Ⅰ/胶原Ⅲ总IOD可降至0.28,与空白组相比有非常显著的差异(P<0.01),补肾活血方组则明显高于模型组(P<0.01),与空白组相比无统计学差异;结论:补肾活血方组可以明显降低BPH模型大鼠膀胱组织胶原Ⅲ沉积,提高胶原I/胶原III比率,这可能是补肾活血方有效改善BPH继发膀胱改变的机制之一。

Objective ：To observe the effect of BushenHuoxue Fang of deposition of collagen in rat bladder tissue. Materials and methods： Clean grade 48 male Wistar rats were randomly divided into normal group, model group, the model plus BushenHuoxue Fang group, model plus Tamslusin group of four groups. Except the normal group, the other rats received castration plus estosterone injection to make model of BPH. On the first day of castration, the rats were given the appropriate medication orally. Model cents a soup by 1 mL/100 g/day for body weight cents a soup （from the Epimedium, Wolfberry, pangolins, Salvia, dodder seed, etc. , including Health and dose 2.34 g/mL） fed group model plus tamslusin by 1.8 μg/100 g/day body weight for tamsulosin orally. Other groups to an equal volume of normal saline. Were 1 day, 38 consecutive days to detect the rat prostate, bladder wet weight and organ index, immunohistochemical observation of bladder tissue collagen deposition. Results ：The model group Ⅲ collagen positive area rate of the bladder, the total IOD etc significantly higher than the control group （P 〈 0.01 ）. Cents a soup, bladder tamsulosin group ]]I collagen positive area rate and the total IOD were significantly lower than model group （ P 〈 0.01 ）. Bladder collagen deposition and collagen Ⅲ I similar. Model group, the bladder collagen I / total IOD can be reduced collagen mo. 28, compared with the control group there was a significant difference （ P 〈 0.01 ）, cents a soup group was significantly higher than model group （P 〈 0.01 ）, with the control group no significant difference; Conclusion： Sin a decoction group of rats can significantly reduce BPH bladder tissue deposition of collagen iii, improve collagen i /III collagen ratio, which may be effective in improving BPH cents a soup to change the mechanism of secondary bladder one.