四磨汤对慢传输结肠五羟色胺兴奋性受体的干预研究

Effect of Simo Decoction on the Expressions of Excitatory Receptors of Serotonin in Slow Transit Colon

目的:研究四磨汤对慢传输结肠5-HT信号系统兴奋性受体5-HT3、4的影响,探索理气中药改善结肠动力的作用机制。方法:选取SD大鼠,采用大黄灌胃法制造结肠慢传输型便秘模型,造模成功后,将36只大鼠再随机分为3组,一组为对照组灌蒸馏水,另两组为四磨汤干预高剂量组和低剂量组。中药干预组灌服四磨汤,连续灌服2周后处死,测炭末推进率,取出标本。Realtime-PCR检测5-HT3、4受体mRNA的表达,免疫组化检测石蜡切片5-HT3、4受体蛋白表达,计算机辅助图像分析表达情况。结果:四磨汤干预高剂量组及低剂量组大鼠炭末推进率高于对照组(P<0.05);两组大鼠结肠中5-HT3、5-HT4受体mRNA表达的ct值均高于对照组(P<0.05),高剂量组与低剂量组之间mRNA表达差异无统计学意义(P>0.05);四磨汤干预高剂量组及低剂量组大鼠结肠中5-HT3、5-HT4受体蛋白的灰度值均高于对照组(P<0.05),高剂量组与低剂量组受体蛋白的表达差异无统计学意义(P>0.05)。结论:四磨汤能够增加慢传输结肠兴奋性受体5-HT3、4的表达量,提示理气中药可能通过上调兴奋性受体5-HT3、4的表达来改善结肠动力。

Objective：To study the effect of Simo Decoction on the expression of excitatory receptors of serotonin in slow transit colon. Method：The animal model with slow transit colon was made by SD rats from garage with rheum officinale. 36 SD rats with slow transit colon were divided into 3 groups ： control group, treatment group with high dosage Sjmo decoction and treatment groUp with iow dosage Simo Decoction. The control group were given distilled water, the treatment group were given Simo decoetion. 2 weeks later, the promote rate of carbon powder was measured and the colon specimen was obtained. 5 - HT3 and 5 - HT4 receptors mRNA and protein expression were detected by real - time PCR and immunohistochemistry, differently. Result ： The promote rate of carbon powder of the two treatment groups were higher than that of the control group（ P 〈0.05）. The ct value of 5 -H33 and 5 -HT4 receptors mRNA of the two treatment groups were higher than those in the control group（ P 〈0.05）. The gray values of 5 -H33 and 5 -HT4 receptors protein of the two treatment groups were higher than those in the control group（P 〈 0.05）. There was on significant difference of the 5 - HT3 and 5 - HT4 receptors expression between treatment group with high dosage Simo Decoction and treatment group with low dosage. Conclusion ： Simo decoction can increase the expressions of 5 - H33 and 5 - HT4 receptors. It seems that Simo Decoction can improve the motive power of colon from upregulating the excitatory receptors of serotonin.