姜黄素对EAE大鼠脊髓中MMP-2、MMP-9表达的影响

Influence of Curcumin on Matrix Metalloproteinase (MMP)-2 and MMP-9 Expressions in Spinal Cord of Experimental Allergic Encephalomyelitis

目的:探讨姜黄素对EAE大鼠脊髓中MMP-2、MMP-9活性的影响。方法:采用豚鼠脊髓匀浆诱导EAE模型,治疗组给予姜黄素进行干预,观察行为学变化,HE染色观察脑组织病理改变,real—time PCR检测颈髓组织MMP-2、MMP-9mRNA表达。结果:与EAE组相比,姜黄素治疗组临床评分明显下降,病程缩短,而且恢复较快;中枢炎性细胞浸润明显减少;MMP-9的转录水平明显下降,两组之间差异具有显著性,而MMP-2的水平两者无明显差异。结论:姜黄素对EAE具有一定的治疗作用,可能与抑制炎症细胞浸润及降低MMP-9的水平有关。

Objective ： To investigate the influence of curcumin on matrix metalloproteinase （MMP） - 2 and MMP - 9 expressions in spinal cord of experimental allergic encephalomyelitis（EAE）. Methods：The animal model was established in SD rats by injecting guinea pig spinal cord homogenate in complete Freund＇s adjuvant （CFA）and bordetella pertussis vaccine. Experimental group was given curcumin, and the changes of clinical symptoms were observed every day. Patho- logical changes of brain were observed by Hematoxylin and Eosin（HE） staining. Real -time PCR was performed to test the transcriptional levels of MMP- 2 and MMP- 9 in cervical cord. Result：Compared with EAE group, the clinical scores and disease course were obviously reduced in curcumin group,furthermore the rats were recuperated quickly. The infiltra- tion of inflammatory cells in central nerval system were obviously lessened. The transcriptional level of MMP - 9 was descend,the discrepancy of EAE and curcumin＇s groups was significant,but the transcriptional level of MMP -2 in the two groups had no difference. Conclusion： Curcumin has therapeutic action on EAE, concerned with the inhibition of inflammatory cell infiltration and reducing the transcriptional level of MMP -9.