基于亚微米光栅透射共振蛋白质检测模型研究

The study of protein-detected model based on submicron transmitting grating resonance

建立了一种基于亚微米光栅透射共振理论的蛋白质检测模型,设计了一种金属光栅型蛋白质检测生物传感器,运用光栅耦合激发表面等离子体共振理论对提出的模型进行了理论分析,通过计算机计算模拟,分析了蛋白质样品折射率、厚度等对透射率的影响。结果表明:透射率与样品折射率、厚度呈二阶多项式关系,并且厚度、折射率都随灵敏度的增加而增加。这种检测模型可应用在蛋白质检测传感方面。基于此方法的蛋白质检测模型具有同时检测蛋白质种类和含量、灵敏度高、实时、无需标记,便于集成芯片化,适用于批量生产、成本低等优点,在蛋白质芯片、生物分析、环境监测、生物器件研发等方面具有广泛的应用前景。

A protein detection model based on submicron transmitting grating resonance is proposed. A middle infrared protein bio-sensor with metal grating is designed by utilizing rigorous coupled-waved analysis method to simulate and analyze the effects of refractive index and thickness to transmittivity. The results indicate that transmittivity has the polynomial relation with refractive index and thickness, and the sensitivity is increased with the thickness, also the sensitivity increasing is more obvious with the increase of refractive index. The detection model can he used to protein sensing. The sensor model has the advantages that the kinds and concentration of protein can be detected at the same time, the sensitivity is high, real-time, free-label, low cost, being convenient for integration and chip, and being suitable for large scale producing. It performs a huge application poten- tial in terms of bio-analysis, environment detection and bio-device designing.